Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ctcf

Cell type

Cell type Class
Neural
Cell type
AtT-20
Tissue
Pituitary
Disease
Tumor

Attributes by original data submitter

Sample

source_name
AtT-20 cells expressing 3x flag-Neo
cell line
AtT-20 cells
cell type
pituitary corticotroph cell
passages
10-20
genotype/variation
expressing 3x flag-Neo
chip antibody
CTCF millipore 07-729

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-400bp were kept for library preparation Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2000 following the manufacturer's protocols to obtain 50bp paired end reads.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
68609075
Reads aligned (%)
97.6
Duplicates removed (%)
8.6
Number of peaks
2502 (qval < 1E-05)

mm9

Number of total reads
68609075
Reads aligned (%)
97.5
Duplicates removed (%)
9.2
Number of peaks
2603 (qval < 1E-05)

Base call quality data from DBCLS SRA