Antigen

Antigen Class

TFs and others

Antigen

Ctcf

Cell type

Cell type Class

Neural

Cell type

AtT-20

Tissue

Pituitary

Disease

Tumor

Sample

source_name

AtT-20 cells expressing 3x flag-Neo

cell line

AtT-20 cells

cell type

pituitary corticotroph cell

passages

10-20

genotype/variation

expressing 3x flag-Neo

chip antibody

CTCF millipore 07-729

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-400bp were kept for library preparation Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2000 following the manufacturer's protocols to obtain 50bp paired end reads.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

68609075

Reads aligned (%)

97.6

Duplicates removed (%)

8.6

Number of peaks

2502 (qval < 1E-05)

mm9

Number of total reads

68609075

Reads aligned (%)

97.5

Duplicates removed (%)

9.2

Number of peaks

2603 (qval < 1E-05)